Application

Single-cell CULTIVATION Unlock new possibilities in cell cultivation

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Single-cell cultivation is a method used to isolate and grow individual cells in controlled environments. However, traditional methods often face challenges in scalability and maintaining optimal growth conditions, leading to inconsistent results. Semi-permeable capsules (SPCs) address these limitations by facilitating continuous nutrient exchange, providing controlled, high-throughput cultivation.

SINGLE-CELL SPHEROID CULTIVATION

Creates 3D cell models for drug screening and disease research, offering better tissue environment representation.

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SINGLE-CELL MICROBIAL CULTIVATION

Enables isolation and growth of microbial strains in controlled or natural environments, preventing competition and allowing enrichment based on growth conditions.

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SPCs - NOVEL BIOREACTORS

SINGLE-CELL SPHEROID CULTIVATION

3D cell cultures, such as spheroids, offer significant advantages over traditional 2D cultures, including improved cell morphology, viability, and in vivo relevance for drug screening and disease modeling. However, both aggregation platforms and droplet microfluidics have limitations in spheroid cultivation.

Single-Cell Cultivation Methods Comparison Table

Semi-permeable capsules (SPCs) address these challenges by facilitating unrestricted diffusion of nutrients and metabolites, supporting scalable, high-throughput spheroid cultivation.

SUSPENSION CELLS

ADHESIVE CELLS

Application Note: High-throughput single-cell cultivation in semi-permeable capsules

Drug Screening

Screen drug candidates and assess their efficacy and toxicity in high-throughput using models that better replicate physiological conditions.

Biobanking

Preserve cells for long-term viability and expand the population upon confirming desired genetic modifications.

Disease Modelling

Replicate disease environments and investigate cellular behaviors and interactions to enhance understanding of disease mechanisms.

SINGLE-CELL MICROBIAL CULTIVATION

Traditional microbial cultivation methods often favor dominant strains, limiting the ability to isolate and analyze less abundant or slow-growing microbes. Single-cell cultivation techniques overcome these challenges by isolating individual bacteria, allowing their growth under controlled or native conditions.

Flux applications

Selective Culturomics

Enrich specific microbes by introducing targeted compounds or modifying environmental conditions that favor the growth of desired microbial populations.

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Unculturable Bacteria Enrichment

Enrich microbes that cannot be cultured under standard lab conditions.

Field Sampling

Preserve microbial communities in the field for later analysis without compromising data integrity.

WORKFLOW

Application Note: High-throughput analysis and enrichment of Semi-Permeable Capsules (SPCs) using FACS

FIELD SAMPLING

SPCs can be used for field sampling to preserve microbial communities and capture genetic material from single-cells, even if cells lyse. This eliminates the need for immediate processing or freezing, allowing flexible analysis without compromising sample integrity.