Single-Microbe DNA Barcoding Kit barcodes up to 10,000 microbial single amplified genomes (SAGs) per experiment, enabling genome recovery for individual cells after sequencing. Start by encapsulating microbial cells from suspension into semi-permeable capsules (SPCs), then seamlessly proceed with lysis, genome amplification, and barcoding. Optimized for Illumina short-read sequencing, the kit can also be adapted to any commercial NGS platform, including short- and long-read sequencing.
| Catalog number | CKP-BARK1 |
| Sufficient for | 3 runs x 10,000 barcoded microbial cells |
| Sample type | Microbial cell suspension |
| For use with (equipment) |
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| Sequencing | Illumina MiSeq, NextSeq 550, NovaSeq |
| Shipping conditions | Dry Ice |
| Storage | -20 °C |
| Intended Use | Research Use Only |
Designed for versatility, our technology seamlessly integrates with diverse microbiomes and isolates, provided that the cells are in aqueous suspension and free from large particulates (>40 μm).
SPCs can withstand harsh lysis conditions, which can be carried out in multiple steps. While the kit includes an alkaline lysis buffer, researchers have the flexibility to use their preferred lysis method, with the exception of mechanical disruption.
Samples can be multiplexed at various stages of the workflow, maximizing efficiency.
Thinking about doing single-cell microbial genomics but not sure whether Single-Microbe DNA Barcoding kit will be a good fit?
Test your sample's full potential quickly and easily by sending it to us. The proof of concept experiment includes the following:
We've partnered with the Single Cell Genomics Center at Bigelow Laboratory to offer advanced bioinformatics services for microbiologists lacking data analysis resources
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