Atrandi at ASHG 2025: Bringing Multiomics Closer to Reality

The American Society of Human Genetics (ASHG) Annual Meeting is one of the largest gatherings of human genetics and genomics researchers in the world. This year, ASHG 2025 will bring thousands of scientists, clinicians, and innovators together in Boston, to share the latest breakthroughs shaping the future of biology and we’re excited to be part of the conversation.

Join Us For Industry Education Session

True Multiomics: Connecting Genotype and Transcriptome at Single-Cell Resolution
When: Wednesday, October 15, 3:00–4:00 PM
Where: Room 204AB

!! Advance Registration: Seating is limited. REGISTER HERE.

Our Chief Scientific Officer, Rapolas Žilionis, PhD, will host an Industry Education Session where we showcase how Semi-Permeable Capsules (SPC) technology unlocks single-cell multiomics.


Session Line-up

Bio: Rapolas Žilionis, PhD, is the Co-founder and Chief Scientific Officer of Atrandi Biosciences. He leads the development of single-cell platforms that enable researchers to map genotype to cell state and function. Under his scientific leadership, Atrandi’s patented Semi-Permeable Capsule (SPC) technology delivers high-throughput, multi-step workflows designed for multiomics at scale.

Talk: A Scalable Platform for Single-Cell Co-Profiling of the Transcriptome and Genotype
Rapolas will introduce our latest DNA–RNA co-sequencing data, demonstrating how SPC-enabled workflows map genotypes directly to transcriptional states, confirm CRISPR edits at target loci, and characterize mutations at single-cell resolution.

Bio: Sanjay Srivatsan is an Assistant Professor in the Basic Sciences Division of the Fred Hutch Cancer Center. He received his undergraduate training at the University of California, Berkeley in Bioengineering, followed by training in the labs of Cole Trapnell, Jay Shendure and David Baker. He now runs a synthetic genomics lab where he builds tools to measure and write genomes.

Talk: Capsule-based Genome Sequencing and Lineage Tracing
As cells divide and age they acquire mutations at a rate of 1-2 per cell division. These mutations, which are stably inherited across generations, could serve as time-calibrated lineage marks; however, detecting these mutations has lagged severely due to their sparsity and a lack of methods for cloning whole genomes en masse. To address these problems we developed a novel semi-permeable capsule (SPC) formulation, capable of isolating single cells and their contents while facilitating biomolecular exchange based on size-selectivity. We demonstrate that SPCs facilitate the simultaneous, clonal expansion of over 200,000 cellular colonies, unbiased nucleic acid amplification, and develop a combinatorial indexing-based single-cell whole genome sequencing (CapWGS) protocol. Using CapWGS we profile cells harboring an ultramutator polymerase ε allele (POLE P286R), which increases the mutation rate per cell division by three orders of magnitude. Sequencing 1000 single-cell genomes from POLE P286R harboring cells, facilitates the recovery of distributed lineage marks, enables the reconstruction of time-calibrated cellular genealogies and reveals a rapid, recurrent and adaptive response to ultramutation through whole genome amplification. These capsule-based approaches establish a framework for the detection and reconstruction of time-calibrated lineages through the cloning and sequencing of ultramutated cells from culture or organisms.

Bio: Yuriy Baglaenko is a research scientist working at the intersection of immunology, genetics and genomics. His group builds new approaches to unlock the mysteries of inflammatory and autoimmune diseases. Their research is a mixture of experimental and computational work that generates and analyzes large-scale genomic datasets.

Talk: The Power of Simultaneous Single Cell RNA and Genomic DNA Sequencing in CRISPR Experiments
Genome wide association studies have identified thousands of mutations in predominantly non-coding DNA that that are associated with polygenic diseases. To functionally investigate these non-coding variants, CRISPR genomic editing in primary human cells is required. However, genomic editing outcomes are unpredictable which limits applicability. In recent work, we developed CRAFTseq, a plate-based methodology for examining CRISPR editing in primary human immune cells. In this work, we show the enhanced power of multi-omic single cell sequencing to detect biologically significant changes in gene and protein expression induced by CRISPR editing. To scale this methodology, we have adapted our protocols to semi-permeable capsules for cell encapsulation and library generation enabling analysis from thousands of cells. Semi-permeable capsules (SPCs) allow for multi-step molecular biology applications with the diffusion of reagents while retaining genomic DNA and RNA products. In this presentation, we share our preliminary work on the capture of genomic DNA (gDNA) and full-length RNA transcriptome using this new technology.

Booth & Posters

We’ll also be at Exhibit Level Halls A–B, Booth #2402. Stop by to learn more about SPC technology, connect with our team, and see how researchers are using it to push single-cell genomics forward.

Scientific posters session details
Poster Number: Board 4095T
NEXT-GENERATION KARYOTYPING: WGA-FREE SINGLE-CELL CNV PROFILING
Presenter: Rapolas Žilionis, PhD
Time/Date: Thursday, October 16, 02:30–04:30 PM

Why it matters
Single-cell analysis is entering a new era. By removing long-standing barriers, SPCs make it possible to connect DNA and RNA data from the same cell at scale. At ASHG 2025, we’re bringing these breakthroughs to the community that needs them most.

Join us in Boston! Let’s shape the future of multiomics together.