- - Excellent genome retention in Semi-Permeable Capsules;
- – Genome coverage uniformity equivalent to industry-leading whole genome amplification (WGA) chemistries;
- – WGA-free sample processing is feasible.
Early access
Application
Single-Cell EUKARYOTIC DNA SEQUENCING Be among the first to leverage large-scale single-cell DNA sequencing
Discover
Recent technological advances have drastically lowered sequencing costs, unlocking new possibilities in human biology and diagnostics. However, sample preparation methods have failed to keep up. The ability to perform high-throughput single-cell DNA sequencing with single-nucleotide resolution and at whole-genome scale has remained out of reach. That is, until now.
 |
 |
 |
|
|---|---|---|---|
| Throughput |
Very high
|
Low
|
Very high
|
| Biological resolution |
Averaged
over populations
|
Single-cell
|
Single-cell
|
| Clonal heterogeneity |
Masked
|
Resolved
|
Resolved
|
| Copy number resolution |
Low
|
High
|
High
|
| Somatic mutations |
Limited detection
|
Resolved
|
Resolved
|
| Phylogeny |
Limited, cannot resolve
lineage relationships
|
Lineage relationships
resolved
|
Lineage relationships
resolved
|
| Cost per cell |
N/A
|
Very High
|
Low
|
Atrandi Biosciences introduces a breakthrough high-throughput single-cell genomic profiling technology, finally making large-scale single-cell DNA sequencing a reality!
OBTAIN HIGH-QUALITY BARCODED SINGLE-CELL GENOMES IN SPCs AND CHOOSE YOUR BIOLOGICAL INQUIRY
Isolate single-cells
1000s – 10,000s cells or nuclei isolated
LYSE
AMPLIFY GENOME
FRAGMENT
BARCODE
Atrandi’s proprietary whole genome amplification technology ensures uniform single-cell genome amplification before being barcoded
Release
Single-cell resolution preserved after releasing into solution
Pooled DNA from single cells can be enriched using commercial or custom DNA hybridization capture panels
No enrichment
Whole Genome Sequencing
Commercial Exome Capture Kits
Whole Exome Sequencing
Commercial or Custom DNA Panels
Targeted Sequencing
Features
Scale to address biological heterogeneity
Process 1000s-10,000s of cells in parallel
HIGH RESOLUTION
Both copy number and single-nucleotide resolution
Uniform genome amplification
Amplify genomes uniformly using Atrandi’s proprietary chemistry
Lower sequencing depth required
Achieve insights with 0.1X to 10X coverage
Low cost for routine use
$0.50 per genome
Flexibility for diverse biological inquiries
WGS, WES or Targeted Sequencing
Applications
CANCER GENOMICS
scDNA sequencing provides insights into tumor heterogeneity, mutation co-occurrence, and clonal evolution, helping to track cancer progression, monitor therapeutic resistance, detect minimal residual disease, and guide personalized treatment strategies.
CELL & GENE THERAPY
Leverage single-cell DNA sequencing to precisely characterize genetic modifications, identify off-target mutations, and track clonal dynamics, enhancing the development and optimization of cell and gene therapies.
DEVELOPMENTAL BIOLOGY
scDNA-seq identifies somatic mutations and genomic variations that shape developmental pathways. It resolves lineage relationships, clarifying how these genetic changes drive cell differentiation and tissue formation while informing therapeutic strategies.
CUSTOM MULTIOMICS ASSAYS
SPCs enable the integration of scDNA-seq with other modalities, allowing researchers to classify cell types and assess gene expression. This combined approach provides insights into how genetic variations drive cellular phenotypes and functional outcomes.
Proof of concept
The proof of concept was tested on the GM12827 (Coriell Institute) human cell line, followed by a challenge study using alcohol-fixed acute myeloid leukemia (AML) cells from four different patients. We present molecular karyotyping data for more than 2,000 individual cells, highlighting the heterogeneous nature of AML genomic architecture.
DIRECT SINGLE-CELL KARYOTYPING
Single-cell karyotyping detects chromosomal abnormalities, such as aneuploidy and structural variations, at the individual cell level, revealing karyotype heterogeneity that bulk methods miss.
SPCs can be used to detect these large-scale chromosomal changes without genome amplification, eliminating biases that can distort results. This direct analysis preserves the native chromosomal landscape, making it ideal for studying genomic instability and genetic alterations in development and disease.
ENRICHMENT STRATEGIES TO DECREASE SEQUENCING COSTS
scWGS can be costly, but with SPC-based enrichment strategies, you can optimize sequencing resources by focusing on the most relevant cells. This approach ensures your sequencing budget is spent efficiently, analyzing only what truly matters.
SPC SELECTION
Cells or genomes of interest are fluorescently labeled and sorted using Atrandi's proprietary system, isolating specific cells for targeted sequencing
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