User-Friendly microfluidic platform for High‑Throughput
Single‑Cell Analysis

Discover

Expand your Research Toolkit with EASY DROPLET & CAPSULE GENERATION AND MANIPULATION

Generate

Inject

Merge

Mix

Store

Split

TECHNICAL NOTE Controllable Droplet Generation Enhances the Accuracy and Sensitivity of Cellular and Molecular Assays

TECHNICAL NOTE Addition of reagents into microfluidic droplets

ONYX COMPONENTS

Onyx Brochure

Onyx Fact Sheet

Syringe Pumps

A combination of 4 independent syringe pumps allows for controlled and precise injection of reagents and samples into microfluidic chips, offering versatility for both complex and routine experiments.

Forcefield Generator

Utilizes a controlled electric field to manipulate droplets within microchannels, enabling active droplet manipulation modules, such as injection or merging.

Embedded UI

Provides users with an intuitive and interactive platform to control and monitor microfluidic experiments across multiple platforms, without the need for software installation.

Microscope Illuminator

An integrated 650nm light source offers controlled, adjustable illumination, enhancing visibility and contrast in microfluidic runs on the Onyx platform without interference with photocleavable compounds.

Wi-Fi antennas

Enable wireless connection to the device providing a convenient means of accessing device software from a distance.

Front

Integrated Microscopy

Enables imaging and tracking of microfluidic processes at a scale of 1μm/px and 1080p resolution, with efficient analysis of particle parameters using automated AI algorithms for feedback on process stability.

High-speed Camera

Records dynamic processes within microfluidic channels at a rate of up to 4000 frames per second, enabling real-time observation of fast-paced events such as droplet formation.

Chip Holder

Securely anchors microfluidic chips during experiments and ensures accurate chip positioning for imaging.

Tube Holder

Securely contains and organizes collection tubes. Compatible with 0.2 mL, 0.5 mL, 1.5 mL and 2.0 mL.

Chip Adapter

Provides a user-friendly snap-on electrical connection to the chip, enabling active droplet manipulation without the need for brittle electrode pins or evaporating liquid electrodes.

Top

Onyx is a microfluidic platform designed to generate and manipulate water-in-oil droplets or semi-permeable capsules for high-throughput single-cell and single-molecule analysis. It offers intuitive syringe pump control, high‑speed microscopy, active manipulation, and image-based droplet analysis—all without locking users into specific assays.

This unparalleled flexibility enables effortless integration, customization, and optimization of any droplet-based workflow. Designed for reliability and ease of use, Onyx is the complete solution for laboratories aiming to redefine liquid handling processes with droplet microfluidics.

Benefits

Convenience

Fully integrated platform with an intuitive user interface.

Flexibility

Unparalleled ability to design or modify any droplet workflow.

Precision

Accurate control and manipulation of small liquid volumes.

High Throughput

Generate 100,000s of droplets for massively parallel experiments.

Technology

Embedded software with AI-assisted experiment control.

Cost Efficiency

Reduced reagent and plastic consumption for economical resource use.

Advantages of droplet microfluidics

Monodisperse encapsulation

Uniform reaction efficiency

High Analysis Throughput & Miniaturization

Increased Effective Concentration

Lower Costs

Single Cell or
Molecule Resolution

Oil–Water
interface

Protein
Engineering

Strain
Engineering

Antibody
Discovery

Advantages of SEMI-PERMEABLE CAPSULES

Multi–Step Workflows

High Throughput

Novel Applications

Liquid Colony
Culture

Microbial
Sequencing

Spheroid
Generation

ONYX APPLICATIONS

PROTEIN ENGINEERING

Protein engineering via directed evolution is a growing field in synthetic biology that can provide new biocatalysts based on predefined industrial or biomedical needs.

Scientific Poster

Application Note

STRAIN ENGINEERING

Strain engineering involves the modification of microbial cells to optimize their genetic and metabolic pathways for specific industrial or research applications

Droplet Microfluidics in Strain Engineering

Individual microbial cells are encapsulated within water-in-oil droplets creating isolated microreactors for massively parallel assays.
Droplet format enables efficient high-throughput screening for desired phenotypes like improved productivity, enhanced bioconversion, or stress resistance, retaining phenotype-genotype connection.

Technology Impact

Facilitates exploration of vast genetic diversity for the development of optimized microbial strains.
Contributes to the development of microbial strains tailored for biotechnological applications.

White Paper

ANTIBODY DISCOVERY

Antibody discovery is the process of identifying new antibodies with a high degree of specificity for a particular target. It typically involves structural computation and library screening.

Droplet Microfluidics in Antibody Discovery

Individual cells expressing unique antibody variants are encapsulated in picoliter-sized droplets allowing for massively parallel assays.
Fluorescence-activated droplet sorting is used for high-throughput screening and selection of cells producing antibodies with desired characteristics.
Droplet format enables the detection of secreted antibodies without the loss of phenotype-genotype connection.

Technology Impact

Contributes to the development of more effective and specific biologics, particularly in fields like immunotherapy and precision medicine.

White Paper

Application Note

Scientific Poster

LIQUID COLONY CULTURE (SPCs)

High-throughput liquid colony culture is an advanced microbiological technique designed for parallel cultivation and screening of numerous microbial colonies in liquid media.

MICROBIAL SEQUENCING (SPCs)

Single-cell microbial sequencing is an advanced genomic technique that enables the parallel analysis of individual microbial cells, allowing for a comprehensive understanding of microbial diversity and heterogeneity.

Scientific Poster

SPHEROID GENERATION (SPCs)

High-throughput spheroid generation is a cutting-edge laboratory technique aimed at efficiently producing three-dimensional cellular structures resembling tissue microenvironments.