Accelerate Your Functional Assay Screening With High Throughput Droplet Sorting

Screen large variant libraries for desired phenotypic qualities quickly and easily with sorting rates as high as 30 kHz, which means completing millions of functional assays in hours compared to days. Fluorescent-activated droplet sorting is compatible with virtually any functional assay where activity can be revealed by a fluorogenic substrate. Droplets containing the desired variants will be sorted and collected for downstream analysis.

Perform high-throughput phenotypic analysis of individual mAb-secreting cells, completing up to millions of single-cell functional screening assays in a single day. Functional mAbs activate reporters to produce a fluorescence signal that triggers the sorting of “positive” droplets from a heterogeneous population and eliminates the need for prior knowledge of binding characteristics. Massive parallelization enables the identification of rare cells that produce high-affinity antibodies.

Fluorescent-activated droplet sorting enables the high-throughput screening of massive DNA-encoded libraries, significantly reducing timelines in early-stage drug discovery. The presence of the desired functional activity can be translated through fluorescence intensity, and droplets containing positive “hits” can be sorted depending on their level of fluorescence. The effects of different drug candidates on various targets and pathways can even be screened simultaneously.

Conduct high-throughput screening of complex environmental samples, including from unculturable bacteria, and sort droplets based on the presence of specific functional activities, such as enzyme activity. Efficient high-throughput screening of large metagenomic libraries enables the identification of less widespread catalytic activities and rare genes that could not be predicted based on sequence homology. Droplet-based functional screening is faster than traditional plate-based assays and also reduces the likelihood of sample contamination.