TECHNOLOGY
Unveiling the
Microbial Universe
with Unprecedented Precision and Scale through Single-Cell Metagenomics
View Product
Discover
High-throughput single-cell metagenomics substantially improves our understanding of the intricate world of unculturable microbes. Shotgun metagenomics and 16S rRNA sequencing, the go-to methods for microbiome characterization, fall short in achieving the level of detail needed for accurate insights.
High-throughput SINGLE-CELL METAGENOMICS
  • Taxonomic resolution: strain-level
  • Functional profiling: yes
  • Linkage with additional DNA: yes
  • Absolute quantification: yes
16S rRNA
sequencing
  • Taxonomic resolution: genus-level
  • Functional profiling: no
  • Linkage with additional DNA: no
  • Absolute quantification: no
Shotgun
metagenomics
  • Taxonomic resolution: species-level
  • Functional profiling: yes
  • Linkage with additional DNA: no
  • Absolute quantification: no
Use Cases
Soil microbiome
Soil contains Earth's most diverse and complex microbiome, with up to 50,000 microbial species per gram.
Marine microbiome
Viruses are the most abundant biological entity in the ocean and infect a wide range of microbial life.
Gut microbiome
Gut microbiome is closely linked to human health and can influence the development of various diseases.
Solution
Single-microbe DNA barcoding kit amplifies and uniquely labels genetic material of 10,000s of individual microbial cells to provide an unmatched resolution into your complex microbiome sample. The recovery of high-quality genomes at scale is possible with our innovative semi-permeable capsule (SPC) technology.
Explore SPC Technology
Workflow
ENCAPSULATION
Isolate individual cells into semi-permeable capsules.
LYSIS AND DNA PURIFICATION
Lyse cells under harsh chemical & enzymatic conditions to access DNA.
GENOME AMPLIFICATION
Amplify whole genomes with MDA optimized for even coverage.
SINGLE CELL LABELLING
Attach unique labels to single amplified genomes (SAGs) by iterative rounds of ligation.
LIBRARY PREPARATION AND SEQUENCING
Process labeled DNA using commercial short-read or long-read library preparation kits.
DATA ANALYSIS
Obtain demultiplexed single amplified genomes (SAGs).
Results
We processed well-characterized E. coli and B. subtilis bacteria. Upon sequencing of ~3,000 cells, important technical metrics, such as cross-contamination and genome recovery, were measured to assess the performance of the workflow.
  • Excellent genome retention in semi-permeable capsules.
  • 90% genome recovery.
  • Absence of systematic bias.
White paper Sample preparation for single-microbe isolation in semi-permeable capsules
Harness the power of high-throughput
single-cell metagenomics using our:
INTUITIVE FLUX
PLATFORM IN
YOUR LAB
Learn More
Or let us test your samples with our kit

Thinking about doing single-cell metagenomics but not sure whether Single-Microbe DNA Barcoding kit will be a good fit?

Test your sample's full potential quickly and easily by sending it to us. The proof of concept experiment includes the following:

  • Sample preparation
  • Cell encapsulation into SPCs
  • Single-microbe DNA barcoding
  • Sequencing on the Illumina platform (NextSeq 550 or NovaSeq)
  • Data demultiplexing by cellular barcodes

For more comprehensive data analysis, please reach out to our partners at the Bigelow Laboratory's SCGC.

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